2. GPCR/G Protein Neuronal Signaling Immunology/Inflammation Cytoskeleton Cell Cycle/DNA Damage
  3. Guanylate Cyclase Monoamine Oxidase NO Synthase Microtubule/Tubulin
  4. Methylene Blue

Methylene Blue  (Synonyms: 亚甲蓝; Basic Blue 9; CI-52015; Methylthioninium chloride)

目录号: HY-14536 纯度: 98.08%
COA 产品使用指南

摩尔计算器

Methylene blue (Basic Blue 9) 是一种鸟苷酸环化酶 (sGC)，单胺氧化酶 A (MAO-A) 和 NO 合酶 (NOS) 抑制剂。Methylene blue 是一种血管加压剂，在医疗中通常用作染料。Methylene blue 通过一氧化氮合成酶/鸟苷酸环化酶信号通路降低脉冲前抑制。Methylene blue 是一种氧化还原循环化合物，能够穿过血脑屏障。Methylene blue 是 Tau 聚集抑制剂。亚甲蓝可减轻脑水肿，减弱小胶质细胞活化，减少神经炎症。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Methylene Blue Chemical Structure

Methylene Blue Chemical Structure

CAS No. : 61-73-4

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规格 价格 是否有货 数量
100 mg ￥500
In-stock
500 mg ￥850
In-stock
1 g   询价  
5 g   询价  

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Methylene Blue 相关抗体

Top Publications Citing Use of Products

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MAO-A MAO-B

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nNOS iNOS eNOS

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Methylene blue (Basic Blue 9) is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue is a vasopressor and is often used as a dye in several medical procedures. Methylene blue through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene blue is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene blue is a Tau aggregation inhibitor. Methylene blue reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation[1][2][3].

IC50 & Target

Guanylyl cyclase (sGC)[1].
Monoamine oxidase A (MAO-A)[1].
NO synthase (NOS)[1]
体外研究
(In Vitro)

Methylene blue (Basic Blue 9) (4.5 μM; BV2 microglia) alters the immune profile of LPS-activated BV2 microglia and decreases the level of CD14, IL-1β, TNF-α, and CCL2 mRNA[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Methylene Blue 相关抗体:
体内研究
(In Vivo)

Methylene blue (Basic Blue 9) (50 and 100 mg/kg; i.p.; once, for 25 min; male NMRI mice) reduces absent prepulse inhibition[1].
Methylene blue (Basic Blue 9) (20 and 40 mg/kg; p.o.; daily, for 6 months; CaMKIIα-tTA transactivator mice) preserves cognition in mice expressing full-length pro-aggregant human Tau[2].
Methylene blue (Basic Blue 9) (2 mg/kg; i.v.; once, for 1 d; TBI-treated male BALB/c mice) reduces TBI-induced edema and neuroinflammation and reduces acute depression-like behavior[3].
Methylene blue (Basic Blue 9) (2 mg/kg; i.v.; once, for 1 d; TBI-treated male BALB/c mice) reduces the percentage of inflammatory factor[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male NMRI mice[1]
Dosage: 50 and 100 mg/kg
Administration: Intraperitoneal injection; once, for 25 minutes
Result: Reduced the prepulse inhibition and reduced the increase in locomotor activity caused by phencyclidine (PCP).
Animal Model: CaMKIIα-tTA transactivator mice[2]
Dosage: 20 and 40 mg/kg
Administration: Oral administration; daily, for 6 months
Result: Inhibited Tau aggregation in CaMKIIα-tTA transactivator mice.
Animal Model: TBI-treated male BALB/c mice[3]
Dosage: 2 mg/kg
Administration: Intravenous injection; once, for 1 day
Result: Decreased the level of CD14, IL-1β, TNF-α, and CCL2 mRNA.
Animal Model: TBI-treated male BALB/c mice[3]
Dosage: 2 mg/kg
Administration: Intravenous injection; once, for 1 day
Result: Reduced the percentage of myeloid (CD11b+/GR1+) cells, reduced IL-1β and enhanced IL-10 expression in microglia.
Clinical Trial
分子量

319.85

Formula

C16H18ClN3S
CAS 号
性状

固体

颜色

Reddish brown to brown

中文名称

亚甲蓝
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Store at room temperature, keep dry and cool
溶解性数据
细胞实验： 

H2O 中的溶解度 : 50 mg/mL (156.32 mM; 超声助溶)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.1265 mL 15.6323 mL 31.2647 mL
5 mM 0.6253 mL 3.1265 mL 6.2529 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
纯度 & 产品资料

纯度: 98.08%

COA (284 KB) HNMR (246 KB) LCMS (263 KB)

产品使用指南 (1538 KB)
染色示例
参考文献

Methylene Blue 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 3.1265 mL 15.6323 mL 31.2647 mL 78.1616 mL
5 mM 0.6253 mL 3.1265 mL 6.2529 mL 15.6323 mL
10 mM 0.3126 mL 1.5632 mL 3.1265 mL 7.8162 mL
15 mM 0.2084 mL 1.0422 mL 2.0843 mL 5.2108 mL
20 mM 0.1563 mL 0.7816 mL 1.5632 mL 3.9081 mL
25 mM 0.1251 mL 0.6253 mL 1.2506 mL 3.1265 mL
30 mM 0.1042 mL 0.5211 mL 1.0422 mL 2.6054 mL
40 mM 0.0782 mL 0.3908 mL 0.7816 mL 1.9540 mL
50 mM 0.0625 mL 0.3126 mL 0.6253 mL 1.5632 mL
60 mM 0.0521 mL 0.2605 mL 0.5211 mL 1.3027 mL
80 mM 0.0391 mL 0.1954 mL 0.3908 mL 0.9770 mL
100 mM 0.0313 mL 0.1563 mL 0.3126 mL 0.7816 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Methylene Blue61-73-4Basic Blue 9 CI-52015Methylthioninium亚甲蓝Basic Blue9Basic Blue-9CI52015CI 52015CI-52015Guanylate CyclaseMonoamine OxidaseNO SynthaseMicrotubule/TubulinMAONitric oxide synthasesNOSvasopressordyeantinociceptionantimalarialantidepressantanxiolyticmethemoglobinemiascGMPInhibitorinhibitorinhibit

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